Apolipoprotein D Transgenic Mice Develop Hepatic Steatosis through Activation of PPAR? and Fatty Acid Uptake

Labrie, Marilyne; Lalonde, Simon; Najyb, Ouafa; Thiery, Maxime; Daneault, Caroline; Des Rosiers, Chrisitne; Rassart, Eric; Mounier, Catherine

Cell culture medium was purchased from Wisent (Wisent, St-Bruno, Qc, Canada). Bodipy 493/503, Prolong Gold antifade reagent, Galacto-light beta-galactosidase reporter gene assay system, Trizol Reagent and mouse anti-myc monoclonal antibody were purchased from Invitrogen (Invitrogen, Burlington, ON, Canada). AA, anti-mouse horseradish peroxidase-conjugated secondary antibody, luciferin and propidium iodure were obtained from Sigma (Sigma-Aldrich, Oakville, ON, Canada). Anti-PPARγ (C26H12), anti-AMPKα, anti-phospho-AMPKα (Thr172)(40H9), HPRT and β-actin antibodies were from Cell signaling (cell signaling technology, Danvers, MA, USA). Anti-ACC and anti-phospho-ACC (Ser79) antibodies were purchased from Millipore (Millipore, Billerica, MA, USA). Anti-Plin2 antibody was bought from Novus Biological (Novus Biologicals, Littleton, CO, USA) and goat anti-rabbit horseradish peroxidase-conjugated secondary antibody and Bradford reagent were acquired from Bio-rad (Life Science Bio-rad, Mississauga, Ontario, Canada). The antibody against mouse apoD was purchased from Abcam. The H-ApoD monoclonal antibody has already been described [7, 43] . Complete Protease Inhibitor Cocktail Tablets were purchased from Roche (Laval, PQ, CAN). Collagenase Type I was acquired from Worthington (Lakewood, NJ). Gal4-PPARγ and UAS-Luciferase plasmids were generously provided by Dr. Maurizio Crestani (University of Milano, Italia).