Author: Barzon, Luisa; Lavezzo, Enrico; Militello, Valentina; Toppo, Stefano; Palù, Giorgio
Title: Applications of Next-Generation Sequencing Technologies to Diagnostic Virology Document date: 2011_11_14
ID: 01nuj0lk_26
Snippet: A recent study [69] developed a bioinformatic annotation strategy for identification and quantitative description of human pathogenic viruses in virome data sets and applied this strategy to annotate sequences of viral DNA and RNA (cDNA) extracted from sewage sludge residuals resulting from municipal wastewater treatment (biosolids), which were obtained by 454 pyrosequencing. In this experimental model, within the 51,925 annotated sequences, 94 D.....
Document: A recent study [69] developed a bioinformatic annotation strategy for identification and quantitative description of human pathogenic viruses in virome data sets and applied this strategy to annotate sequences of viral DNA and RNA (cDNA) extracted from sewage sludge residuals resulting from municipal wastewater treatment (biosolids), which were obtained by 454 pyrosequencing. In this experimental model, within the 51,925 annotated sequences, 94 DNA and 19 RNA sequences were identified as human viruses. Virus diversity included environmentally transmitted agents such as parechovirus, coronavirus, adenovirus and aichi virus, as well as viruses associated with chronic human infections, such as human herpesviruses and hepatitis C virus [69] .
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