Author: Zhai, Xiaofeng; Wang, Shilei; Zhu, Mengyan; He, Wei; Pan, Zhongzhou; Su, Shuo
Title: Antiviral Effect of Lithium Chloride and Diammonium Glycyrrhizinate on Porcine Deltacoronavirus In Vitro Document date: 2019_9_9
ID: 1m5yr8uc_7
Snippet: LLC-PK1 cells were seeded into 12-cell plates and grown to 100% confluence for 24 h. Non-toxic concentrations of LiCl (60 mM) or DG (1250 µg/mL) were mixed with the virus (MOI 0.05), and the mixtures were then added to the cells, followed by incubation for 1 h at 4 • C. As a control, the cells were infected with the same dose of PDCoV without drug treatment. After washing with cold PBS, the levels of vRNA in the cells were determined by real-t.....
Document: LLC-PK1 cells were seeded into 12-cell plates and grown to 100% confluence for 24 h. Non-toxic concentrations of LiCl (60 mM) or DG (1250 µg/mL) were mixed with the virus (MOI 0.05), and the mixtures were then added to the cells, followed by incubation for 1 h at 4 • C. As a control, the cells were infected with the same dose of PDCoV without drug treatment. After washing with cold PBS, the levels of vRNA in the cells were determined by real-time qPCR.
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