Antibody Engineering for Pursuing a Healthier Future

Saeed, Abdullah F. U. H.; Wang, Rongzhi; Ling, Sumei; Wang, Shihua

Phage display panning methods have failed to yield specific results because of the presence of parasitic phage clones that are often not removed by washing. To overcome these limitations, next generation sequencing (NGS) technology is used to sequence sub-libraries in biopanning experiments (Naqid et al., 2016) . Next generation phage display (NGPD) can sequence 3,000 up to a million reads per panning round. This method is more rapid compared with traditional ELISA screening. NGPD has been used to identify target specific ligands by a single panning round using a library or ligand motif (Ravn et al., 2013) . This technique has widespread applications for the identification of thousands of ligands and mapping antibodies in response to infectious particles. Clinical targets and antigenrelated ligands have been discovered with the help of genetically encoded peptide libraries. Correspondingly, ligands are selected using NGPD by the detection of low abundant copy clones without numerous rounds of selection. Subsequently, NGS has facilitated the quantification of gene expression, genome assembly, and metagenome analysis. Additionally, it is used for PDT with the ion torrent technique (Matochko and Derda, 2015) .