Author: Yu, Liping; Zhang, Xiaorong; Wu, Tianqi; Su, Jin; Wang, Yuyang; Wang, Yuexin; Ruan, Baoyang; Niu, Xiaosai; Wu, Yantao
Title: Avian infectious bronchitis virus disrupts the melanoma differentiation associated gene 5 (MDA5) signaling pathway by cleavage of the adaptor protein MAVS Document date: 2017_11_13
ID: 0zn1sqj9_5
Snippet: The JS/2010/12 strain of IBV was previously characterized as nephropathogenic by our laboratory, and its genomic sequence was determined (GenBank accession No. JQ900122.1). In this study, the stock of JS/2010/12 strain propagated in 10-day-old SPF Line 22 of White Leghorn chicken embryos for 5 passages (P5) was used. The 50% tissue culture infective dose (TCID50) of the IBV strain was determined by identifying the cytopathic effect (CPE) induced .....
Document: The JS/2010/12 strain of IBV was previously characterized as nephropathogenic by our laboratory, and its genomic sequence was determined (GenBank accession No. JQ900122.1). In this study, the stock of JS/2010/12 strain propagated in 10-day-old SPF Line 22 of White Leghorn chicken embryos for 5 passages (P5) was used. The 50% tissue culture infective dose (TCID50) of the IBV strain was determined by identifying the cytopathic effect (CPE) induced by the virus in CEK cells. The DF1 chicken fibroblast cell line was used for all transfection-based assays. The cells were maintained in Dulbecco's modified Eagle's medium (DMEM, HyClone) containing 10% FBS. CEK cells were aseptically generated from 20-day-old SPF chicken embryos. The cell suspension was obtained by trypsinization of kidneys for 30 min at 37°C and subsequent filtration with a 100-μm mesh. Then, the cells were cultured in M199 media (HyClone) containing 3% FBS (HyClone).
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