Selected article for: "fluorescence microscope and immunofluorescence assay"

Author: Chen, Shilong; Wang, Long; Chen, Jieying; Zhang, Lanlan; Wang, Song; Goraya, Mohsan U.; Chi, Xiaojuan; Na, Yang; Shao, Wenhan; Yang, Zhou; Zeng, Xiancheng; Chen, Shaoying; Chen, Ji-Long
Title: Avian Interferon-Inducible Transmembrane Protein Family Effectively Restricts Avian Tembusu Virus Infection
  • Document date: 2017_4_20
  • ID: 0pjg25kn_17
    Snippet: Indirect immunofluorescence assay was performed as previously described (Chen et al., 2016b) . Briefly, the parenchymal organs sections and DEF cells infected with ATMUV were incubated with mouse anti-ATMUV E protein monoclonal antibody for 30 min at 37 • C and then washed with PBS, followed by incubation with FITC conjugated goat anti-mouse IgG at 37 • C for 30 min before imaging with fluorescence microscope (Nikon, Japan)......
    Document: Indirect immunofluorescence assay was performed as previously described (Chen et al., 2016b) . Briefly, the parenchymal organs sections and DEF cells infected with ATMUV were incubated with mouse anti-ATMUV E protein monoclonal antibody for 30 min at 37 • C and then washed with PBS, followed by incubation with FITC conjugated goat anti-mouse IgG at 37 • C for 30 min before imaging with fluorescence microscope (Nikon, Japan).

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