Author: Zhai, Xiaofeng; Wang, Shilei; Zhu, Mengyan; He, Wei; Pan, Zhongzhou; Su, Shuo
Title: Antiviral Effect of Lithium Chloride and Diammonium Glycyrrhizinate on Porcine Deltacoronavirus In Vitro Document date: 2019_9_9
ID: 1m5yr8uc_17
Snippet: Apoptosis was investigated using the AnnexinV Alexa Fluor647/PI kit (FMSAV647-100) by flow cytometry. LLC-PK1 cells were detached using Accutase Cell Detachment Solution (FMS-AT 100) for 5 min and then centrifuged at 2000 rpm for 5 min. The cells were washed twice with PBS and re-suspended with 500 µL of binding buffer at a concentration of 10 6 cells/mL. Then, 5 µL of AF647-conjugated AnnexinV and 10 µL of PI were added to the cells, which wa.....
Document: Apoptosis was investigated using the AnnexinV Alexa Fluor647/PI kit (FMSAV647-100) by flow cytometry. LLC-PK1 cells were detached using Accutase Cell Detachment Solution (FMS-AT 100) for 5 min and then centrifuged at 2000 rpm for 5 min. The cells were washed twice with PBS and re-suspended with 500 µL of binding buffer at a concentration of 10 6 cells/mL. Then, 5 µL of AF647-conjugated AnnexinV and 10 µL of PI were added to the cells, which was followed by incubation at room temperature for 15 min in the dark. Flow cytometry was carried out in a Becton Dickinson Accuri C6 FACSCalibur cytometer (BD Accuri C6 Plus, BD Medical Devices (Shanghai) Co., Ltd., Shanghai, China).
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