Author: Labrie, Marilyne; Lalonde, Simon; Najyb, Ouafa; Thiery, Maxime; Daneault, Caroline; Des Rosiers, Chrisitne; Rassart, Eric; Mounier, Catherine
Title: Apolipoprotein D Transgenic Mice Develop Hepatic Steatosis through Activation of PPAR? and Fatty Acid Uptake Document date: 2015_6_17
ID: 0wtq1c15_11
Snippet: Tissues were collected, frozen in dry ice and kept at −80°C until further use. Total RNA was extracted with the TRIZOL reagent according to the manufacturer instructions. Total RNA was then reverse transcribed using Transcriptor First Strand cDNA Synthesis Kit and amplified with a Taq DNA polymerase and specific primers (S1 Table) . HPRT was used as control......
Document: Tissues were collected, frozen in dry ice and kept at −80°C until further use. Total RNA was extracted with the TRIZOL reagent according to the manufacturer instructions. Total RNA was then reverse transcribed using Transcriptor First Strand cDNA Synthesis Kit and amplified with a Taq DNA polymerase and specific primers (S1 Table) . HPRT was used as control.
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