Selected article for: "ecl GE Healthcare prime chemiluminescence reagent and GE Healthcare prime chemiluminescence reagent"
Author: Yong, Kylie Su Mei; Ng, Justin Han Jia; Her, Zhisheng; Hey, Ying Ying; Tan, Sue Yee; Tan, Wilson Wei Sheng; Irac, Sergio Erdal; Liu, Min; Chan, Xue Ying; Gunawan, Merry; Foo, Randy Jee Hiang; Low, Dolyce Hong Wen; Mendenhall, Ian Hewitt; Chionh, Yok Teng; Dutertre, Charles-Antoine; Chen, Qingfeng; Wang, Lin-Fa
Title: Bat-mouse bone marrow chimera: a novel animal model for dissecting the uniqueness of the bat immune system
  • Document date: 2018_3_16
    • ID: 01f36rld_26
    Snippet: Western blot. Specificity of bat immunoglobulin G (IgG) was determined using western blot. Pooled sera from two individual bats (E. spelaea) and sera from C57BL/6 mice, both diluted 1:50 in PBS, were ran on a 12% SDS-PAGE gel, followed by transfer onto Polyvinylidene difluoride (PVDF) transfer membranes (Millipore, USA). Membranes were blocked in Tris-buffered saline with 0.1% Tween (TBS-T 0.1%) and 2.5% skim milk for 1 hour at room temperature (.....
    Document: Western blot. Specificity of bat immunoglobulin G (IgG) was determined using western blot. Pooled sera from two individual bats (E. spelaea) and sera from C57BL/6 mice, both diluted 1:50 in PBS, were ran on a 12% SDS-PAGE gel, followed by transfer onto Polyvinylidene difluoride (PVDF) transfer membranes (Millipore, USA). Membranes were blocked in Tris-buffered saline with 0.1% Tween (TBS-T 0.1%) and 2.5% skim milk for 1 hour at room temperature (RT), followed by labelling with goat anti-bat IgG heavy and light chain antibody (A140-118B; Bethyl Laboratories, USA) diluted 1:1,000 in TBS-T 0.1% overnight at 4 °C. Membrane was washed 3 times for 5 minutes with TBS-T 0.1%, then labelled with donkey anti-goat IgG-HRP (SC-2056; Santa Cruz Biotechnology, USA) diluted 1:10,000 in TBS-T 0.1% at RT for 1 hour, and subsequently undergo a final 3 times 5 minutes wash. Membrane was visualized using enhanced chemiluminescence (ECL) prime chemi-luminescence reagent (GE Healthcare, USA) and a myECL Imager (Thermo Fisher Scientific, USA).

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