Selected article for: "high throughput and sequence length"
Author: Barzon, Luisa; Lavezzo, Enrico; Militello, Valentina; Toppo, Stefano; Palù, Giorgio
Title: Applications of Next-Generation Sequencing Technologies to Diagnostic Virology
  • Document date: 2011_11_14
    • ID: 01nuj0lk_3
    Snippet: These NGS methods have different underlying biochemistries and differ in sequencing protocol (sequencing by synthesis for 454 pyrosequencing, Illumina GA, Ion Torrent PGM and Heliscope, sequencing by ligation for SOLiD), throughput, and for sequence length (Table 1) . Thus, the SOLiD system may be more suitable for applications that require a very high throughput of sequences, but not long reads, such as whole genome re-sequencing or RNA-sequenci.....
    Document: These NGS methods have different underlying biochemistries and differ in sequencing protocol (sequencing by synthesis for 454 pyrosequencing, Illumina GA, Ion Torrent PGM and Heliscope, sequencing by ligation for SOLiD), throughput, and for sequence length (Table 1) . Thus, the SOLiD system may be more suitable for applications that require a very high throughput of sequences, but not long reads, such as whole genome re-sequencing or RNA-sequencing projects, while both 454 and Illumina provide data suitable for de novo assembly and the relative long length of 454 FLX (and its smaller version GS Junior) reads allows deep sequencing of amplicons, with applications in microbial and viral metagenomics and analysis of viral quasispecies, as described in this review. The technical features of NGS methods (reviewed in refs. [2, 3] ) will not be described in this review, which is focused on the diagnostic applications of NGS in clinical virology.

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