Selected article for: "loading control protein and lysis buffer"
Author: Yu, Liping; Zhang, Xiaorong; Wu, Tianqi; Su, Jin; Wang, Yuyang; Wang, Yuexin; Ruan, Baoyang; Niu, Xiaosai; Wu, Yantao
Title: Avian infectious bronchitis virus disrupts the melanoma differentiation associated gene 5 (MDA5) signaling pathway by cleavage of the adaptor protein MAVS
  • Document date: 2017_11_13
    • ID: 0zn1sqj9_15
    Snippet: CEK cells were infected with IBV, and at different time points post-infection, the cells were lysed with RIPA lysis buffer (Beyotime Institute of Biotechnology, China). The cell lysates were analyzed for N proteins by Western blot with anti-N antibody (1:1000) (Prepared by our laboratory). Actin was detected using a β-actin antibody (1:5000, Sigma) as a protein loading control. An anti-MAVS antibody (1:1000, Cell Signaling Technology) was used t.....
    Document: CEK cells were infected with IBV, and at different time points post-infection, the cells were lysed with RIPA lysis buffer (Beyotime Institute of Biotechnology, China). The cell lysates were analyzed for N proteins by Western blot with anti-N antibody (1:1000) (Prepared by our laboratory). Actin was detected using a β-actin antibody (1:5000, Sigma) as a protein loading control. An anti-MAVS antibody (1:1000, Cell Signaling Technology) was used to detect the MAVS protein.

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