Author: Klaile, Esther; Klassert, Tilman E; Scheffrahn, Inka; Müller, Mario M; Heinrich, Annina; Heyl, Kerstin A; Dienemann, Hendrik; Grünewald, Christiane; Bals, Robert; Singer, Bernhard B; Slevogt, Hortense
Title: Carcinoembryonic antigen (CEA)-related cell adhesion molecules are co-expressed in the human lung and their expression can be modulated in bronchial epithelial cells by non-typable Haemophilus influenzae, Moraxella catarrhalis, TLR3, and type I and II interferons Document date: 2013_8_14
ID: 1fmsipqu_48
Snippet: Also, an increased CEACAM5 cell surface expression upon IFNγ treatment could be confirmed by FACS analysis ( Figure 4C ). Flow cytometry also verified that there was only a small subpopulation of NHBE cells that were CEACAM5 positive in untreated control cells. Upon IFNγ treatment, both, the number of CEACAM5positive cells and the expression level of CEACAM5 on the cell surface, were increased ( Figure 4C ). However, it remained only a defined .....
Document: Also, an increased CEACAM5 cell surface expression upon IFNγ treatment could be confirmed by FACS analysis ( Figure 4C ). Flow cytometry also verified that there was only a small subpopulation of NHBE cells that were CEACAM5 positive in untreated control cells. Upon IFNγ treatment, both, the number of CEACAM5positive cells and the expression level of CEACAM5 on the cell surface, were increased ( Figure 4C ). However, it remained only a defined portion of NHBE cells that expressed CEACAM5. Structure of forward and reverse primers (5′-3′) of all target genes and isoforms and the expected size of their respective amplified PCR-products.
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