Selected article for: "bovine serum and confocal microscope"
Author: Klaile, Esther; Klassert, Tilman E; Scheffrahn, Inka; Müller, Mario M; Heinrich, Annina; Heyl, Kerstin A; Dienemann, Hendrik; Grünewald, Christiane; Bals, Robert; Singer, Bernhard B; Slevogt, Hortense
Title: Carcinoembryonic antigen (CEA)-related cell adhesion molecules are co-expressed in the human lung and their expression can be modulated in bronchial epithelial cells by non-typable Haemophilus influenzae, Moraxella catarrhalis, TLR3, and type I and II interferons
  • Document date: 2013_8_14
    • ID: 1fmsipqu_23
    Snippet: NHBE cells were grown on cover slips, fixed in PBS/4% paraformaldehyde and blocked with PBS/10% bovine serum albumin. Confocal images of indirect immunofluorescence of CEACAM1, CEACAM5 and CEACAM6 (B3-17, 2H8-5, and 16H10, respectively, and Alexa Fluor 488-conjugated goat anti-mouse IgG), actin (TRIC-conjugated phalloidin) and nuclei (Hoechst33342) were taken using a Zeiss 710 confocal microscope equipped with the Plan-APO 40X/1.4 Oil objective. .....
    Document: NHBE cells were grown on cover slips, fixed in PBS/4% paraformaldehyde and blocked with PBS/10% bovine serum albumin. Confocal images of indirect immunofluorescence of CEACAM1, CEACAM5 and CEACAM6 (B3-17, 2H8-5, and 16H10, respectively, and Alexa Fluor 488-conjugated goat anti-mouse IgG), actin (TRIC-conjugated phalloidin) and nuclei (Hoechst33342) were taken using a Zeiss 710 confocal microscope equipped with the Plan-APO 40X/1.4 Oil objective. Pictures were processed using the Zen Software (Zeiss) and Adobe Photoshop.

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