Author: Klaile, Esther; Klassert, Tilman E; Scheffrahn, Inka; Müller, Mario M; Heinrich, Annina; Heyl, Kerstin A; Dienemann, Hendrik; Grünewald, Christiane; Bals, Robert; Singer, Bernhard B; Slevogt, Hortense
Title: Carcinoembryonic antigen (CEA)-related cell adhesion molecules are co-expressed in the human lung and their expression can be modulated in bronchial epithelial cells by non-typable Haemophilus influenzae, Moraxella catarrhalis, TLR3, and type I and II interferons Document date: 2013_8_14
ID: 1fmsipqu_16
Snippet: Immunoprecipitations from cell culture supernatants NHBE cell culture supernatants from confluent cells were harvested after 48 h. CEACAM1, CEACAM5 and CEACAM6 were precipitated from 10 ml supernatant using 20 μl protein G-Sepharose 4 Fast Flow (GE Healthcare) and 3 μg of monoclonal antibodies B3-17, 2H8-5, and 13H10, respectively. An IgG1 isotype control was included. Immunoprecipitations were analyzed by Western blot using anti CEA polyclonal.....
Document: Immunoprecipitations from cell culture supernatants NHBE cell culture supernatants from confluent cells were harvested after 48 h. CEACAM1, CEACAM5 and CEACAM6 were precipitated from 10 ml supernatant using 20 μl protein G-Sepharose 4 Fast Flow (GE Healthcare) and 3 μg of monoclonal antibodies B3-17, 2H8-5, and 13H10, respectively. An IgG1 isotype control was included. Immunoprecipitations were analyzed by Western blot using anti CEA polyclonal antibody that cross-reacts with all three CEACAMs and HRP-coupled goat anti-rabbit IgG. Analysis was done using the Fusion FX System (Peqlab) and SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific).
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