Aurintricarboxylic Acid Is a Potent Inhibitor of Influenza A and B Virus Neuraminidases

Hashem, Anwar M.; Flaman, Anathea S.; Farnsworth, Aaron; Brown, Earl G.; Van Domselaar, Gary; He, Runtao; Li, Xuguang

Next we tested the effect of ATA on a common oseltamivirresistant viral strain, i.e. WSN (H1N1) virus with the substitution at H274Y, in comparison with the parental wild-type WSN H1N1 virus [33] . As shown in Fig. 10A and 10B , ATA inhibited replication of both WSN and H274Y viruses in a dose-responsive fashion. The IC 50 values obtained for ATA inhibition of WSN and H274Y viruses were 2 mg/ml and 18 mg/ml, respectively (Fig. 10B) . Although the H274Y mutation significantly increased ATA IC 50 by 9-folds as compared to the wild-type virus, ATA remains a potent inhibitor of both wild-type WSN and H274Y oseltamivir-resistant WSN when compared to the 754-fold increase in IC 50 of oseltamivir in inhibiting the H274Y mutant [33] .