Author: Hashem, Anwar M.; Flaman, Anathea S.; Farnsworth, Aaron; Brown, Earl G.; Van Domselaar, Gary; He, Runtao; Li, Xuguang
Title: Aurintricarboxylic Acid Is a Potent Inhibitor of Influenza A and B Virus Neuraminidases Document date: 2009_12_17
ID: 13bvkj2t_6
Snippet: Madin-Darby canine kidney (MDCK) cells (ATCC: CCL-34) were obtained from the American Type Culture Collection (Manassas, VA, USA) and were grown in modified minimum essential medium (modified MEM) containing Earle's balanced salts and supplemented with 2 mM L-glutamine, 1.5 g/l sodium bicarbonate (pH 7.2), 0.1 mM non-essential amino acids, 1.0 mM sodium pyruvate, 10% heat-inactivated fetal bovine serum (FBS), 100 U/ml penicillin and 100 mg/ml str.....
Document: Madin-Darby canine kidney (MDCK) cells (ATCC: CCL-34) were obtained from the American Type Culture Collection (Manassas, VA, USA) and were grown in modified minimum essential medium (modified MEM) containing Earle's balanced salts and supplemented with 2 mM L-glutamine, 1.5 g/l sodium bicarbonate (pH 7.2), 0.1 mM non-essential amino acids, 1.0 mM sodium pyruvate, 10% heat-inactivated fetal bovine serum (FBS), 100 U/ml penicillin and 100 mg/ml streptomycin (Invitrogen, Carlsbad, CA) in a humidified atmosphere of 5% CO 2 . All viruses were amplified and titrated in MDCK cells and stored at 280uC until use.
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