Characterization of a novel orthoreovirus isolated from fruit bat, China

Hu, Tingsong; Qiu, Wei; He, Biao; Zhang, Yan; Yu, Jing; Liang, Xiu; Zhang, Wendong; Chen, Gang; Zhang, Yingguo; Wang, Yiyin; Zheng, Ying; Feng, Ziliang; Hu, Yonghe; Zhou, Weiguo; Tu, Changchun; Fan, Quanshui; Zhang, Fuqiang

PCRs testing were repeated on the 50 fruit bats original samples including the Kidney, heart, lung, liver, spleen, intestine, rectal swab sample, and brain samples. Two bat's QPCRs results were positive. One bat's QPCRs result was positive in the lung, intestine sample (Cangyuan virus isolated) and rectal swab sample, and the Ct (Threshold Cycle) of QPCR were 19.86 ± 0.056, 19.52 ± 0.041, 19.64 ± 0.061 respectively. The Ct of another bat's PCR were 23.07 ± 0.253, 22.53 ± 0.171 in the intestine sample and rectal swab sample, respectively. To establish the evolutionary relationship between Cangyuan virus and other known orthoreoviruses, Homology were compared ( Table 2, Table 3 and Additional file 1: Table S1 , Additional file 2: Table S2 and Additional file 3: Table S3 ) and phylogenetic trees were constructed based on the nucleotide sequences of the L genome segments (Figure 2 ), the M genome segments ( Figure 3 ) and the S genome segments (Figure 4 ). The Cangyuan virus L1-L3, M1-M3 segments sequence identity were 81.6% -94.2%, 83.8%-97.9%, 85.9%-97.6% ( Additional file 1: Table S1 ), 82.2%-94.1%, 78. 1%-95.0%, and 83.0%-93.9% (Table 2 , Additional file 2: Table 2 ), respectively, by alignment with Pteropine orthoreovirus (PRV) species group. The phylogenetic trees for L2, L3, M1 and M2 segments demonstrated that Cangyuan virus was most closely related to Melaka and Kampar viruses, and was placed in Pteropine orthoreovirus (PRV) species group which covers all known bat-borne orthoreoviruses together with Nelson Bay orthoreovirus [12, 15, 21] .