Characterization of a Novel RNA Virus Discovered in the Autumnal Moth Epirrita autumnata in Sweden

de Miranda, Joachim R.; Hedman, Harald; Onorati, Piero; Stephan, Jörg; Karlberg, Olof; Bylund, Helena; Terenius, Olle

Epirrita autumnata field-samples, as determined by four matching pairs of strand-specific RT-qPCR assays: negative-strand assays 1 to 4 (neg1~neg4) and its corresponding positive-strand assays 1 to 4 (pos1~pos4). The data refer to estimated copies cDNA per qPCR reaction. The lighter colored bars in each series refer to the assays located in ORF-1 (genomic region; (g)) while the darker colored bars refer to assays located in ORF-2 and ORF-3 (genomic + sub-genomic region; (g + sg)). The dimmed areas mark the detection thresholds for the assays at Cq = 35. The error bars mark the standard deviation for the assay based on triplicate runs. Samples 32A, 61A, 78A, and 86A are viruspositive adult E. autumnata; sample 7L is the original virus-positive E. autumnata larva; sample 25A is a virus-negative adult E. autumnata. The no-template cDNA controls and no-template qPCR controls were negative for all assays. Figure 2 . Amounts of negative-strand (blue bars) and positive-strand (orange bars) Abisko virus RNA in different Epirrita autumnata field-samples, as determined by four matching pairs of strand-specific RT-qPCR assays: negative-strand assays 1 to 4 (neg1~neg4) and its corresponding positive-strand assays 1 to 4 (pos1~pos4). The data refer to estimated copies cDNA per qPCR reaction. The lighter colored bars in each series refer to the assays located in ORF-1 (genomic region; (g)) while the darker colored bars refer to assays located in ORF-2 and ORF-3 (genomic + sub-genomic region; (g + sg)). The dimmed areas mark the detection thresholds for the assays at Cq = 35. The error bars mark the standard deviation for the assay based on triplicate runs. Samples 32A, 61A, 78A, and 86A are virus-positive adult E. autumnata; sample 7L is the original virus-positive E. autumnata larva; sample 25A is a virus-negative adult E. autumnata. The no-template cDNA controls and no-template qPCR controls were negative for all assays.