Selected article for: "anti antibody and total protein"
Author: Perra, Léa; Balloy, Viviane; Foussignière, Tobias; Moissenet, Didier; Petat, Hortense; Mungrue, Imran N.; Touqui, Lhousseine; Corvol, Harriet; Chignard, Michel; Guillot, Loic
Title: CHAC1 Is Differentially Expressed in Normal and Cystic Fibrosis Bronchial Epithelial Cells and Regulates the Inflammatory Response Induced by Pseudomonas aeruginosa
  • Document date: 2018_11_29
    • ID: 10fe70kl_15
    Snippet: Total proteins were extracted using a NucleoSpin RNA/Protein kit (Macherey Nagel). An equal amount of proteins was reduced, size-separated on 10 or 13.5% SDS-polyacrylamide gels, and transferred to nitrocellulose membranes (Invitrogen). The membranes were blocked in 5% milk in TBS-Tween 0.1% and incubated with specific primary antibodies against CHAC1 (AV42623; Sigma-Aldrich), PARP (9542; Cell Signaling Technology (CST), Danvers, MA, USA), ATF4 (.....
    Document: Total proteins were extracted using a NucleoSpin RNA/Protein kit (Macherey Nagel). An equal amount of proteins was reduced, size-separated on 10 or 13.5% SDS-polyacrylamide gels, and transferred to nitrocellulose membranes (Invitrogen). The membranes were blocked in 5% milk in TBS-Tween 0.1% and incubated with specific primary antibodies against CHAC1 (AV42623; Sigma-Aldrich), PARP (9542; Cell Signaling Technology (CST), Danvers, MA, USA), ATF4 (11815; CST), cleaved caspase-3 (9661; CST), phospho-and total NF-κB-p65 (S536 and D14E12; CST), and β-actin (A2228; Sigma-Aldrich). For anti-CHAC1 antibody validation, CHAC1 overexpression was performed with a CHAC1-V5eGFP plasmid (CHAC1-GFP), as previously shown (11

    Search related documents:
    Co phrase search for related documents
    • antibody validation and total protein: 1
    • Invitrogen membrane and primary antibody: 1
    • Invitrogen membrane and total protein: 1
    • Macherey Nagel Protein kit and NucleoSpin RNA Macherey Nagel Protein kit: 1
    • Macherey Nagel Protein kit and Protein kit: 1
    • Macherey Nagel Protein kit and RNA Macherey Nagel Protein kit: 1
    • NucleoSpin RNA Macherey Nagel Protein kit and Protein kit: 1
    • NucleoSpin RNA Macherey Nagel Protein kit and RNA Macherey Nagel Protein kit: 1
    • previously show and sds polyacrylamide: 1
    • previously show and sds polyacrylamide gel: 1
    • primary antibody and protein equal: 1, 2, 3, 4, 5
    • primary antibody and Protein kit: 1, 2, 3
    • primary antibody and sds polyacrylamide: 1, 2, 3, 4, 5, 6
    • primary antibody and sds polyacrylamide gel: 1, 2, 3, 4, 5, 6
    • primary antibody and total phospho: 1