Author: Chen, Shilong; Wang, Long; Chen, Jieying; Zhang, Lanlan; Wang, Song; Goraya, Mohsan U.; Chi, Xiaojuan; Na, Yang; Shao, Wenhan; Yang, Zhou; Zeng, Xiancheng; Chen, Shaoying; Chen, Ji-Long
Title: Avian Interferon-Inducible Transmembrane Protein Family Effectively Restricts Avian Tembusu Virus Infection Document date: 2017_4_20
ID: 0pjg25kn_19
Snippet: DF-1 cells were lysed with lysis buffer containing 1 × complete protease inhibitor cocktail for 30 min on ice according to our previous method (Wang et al., 2014) . Cell lysates were separated on 12% SDS-PAGE gels, transferred to PVDF membranes and blocked with 5% (w/v) milkpowder in Tris-buffered saline (pH7.4, TBS) for 2 h at room temperature. The membranes were incubated with indicated primary antibodies for 2.5 h at room temperature and wash.....
Document: DF-1 cells were lysed with lysis buffer containing 1 × complete protease inhibitor cocktail for 30 min on ice according to our previous method (Wang et al., 2014) . Cell lysates were separated on 12% SDS-PAGE gels, transferred to PVDF membranes and blocked with 5% (w/v) milkpowder in Tris-buffered saline (pH7.4, TBS) for 2 h at room temperature. The membranes were incubated with indicated primary antibodies for 2.5 h at room temperature and washed with TBS, followed by incubation with appropriate secondary antibodies at room temperature before imaging with the ProteinSimple FluorChem M system (Bio-Techne, USA).
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