Author: Hu, Tingsong; Qiu, Wei; He, Biao; Zhang, Yan; Yu, Jing; Liang, Xiu; Zhang, Wendong; Chen, Gang; Zhang, Yingguo; Wang, Yiyin; Zheng, Ying; Feng, Ziliang; Hu, Yonghe; Zhou, Weiguo; Tu, Changchun; Fan, Quanshui; Zhang, Fuqiang
Title: Characterization of a novel orthoreovirus isolated from fruit bat, China Document date: 2014_11_30
ID: 1fw9ng2p_6
Snippet: The Vero-E6 cells showed a syncytial cytopathic effect (CPE) after 24 hours of the first inoculation at 37°C ( Figure 1 ). The virus was named "Cangyuan virus" after the location from which the host bats (Rousettus leschenaultia) were collected (Cangyuan city of Yunnan province, China). After the first passage in Vero E6 cells, Cangyuan virus began to cause syncytial CPE 24 hours post-inoculation; notably earlier than for Melaka virus and other .....
Document: The Vero-E6 cells showed a syncytial cytopathic effect (CPE) after 24 hours of the first inoculation at 37°C ( Figure 1 ). The virus was named "Cangyuan virus" after the location from which the host bats (Rousettus leschenaultia) were collected (Cangyuan city of Yunnan province, China). After the first passage in Vero E6 cells, Cangyuan virus began to cause syncytial CPE 24 hours post-inoculation; notably earlier than for Melaka virus and other orthoreovirus ( Figure 1B) [9, 16, 17, 20] . QPCR analysis demonstrated that the replication of Cangyuan virus began after 12 hours infected the Vero E6 cells ( Figure 1C ).
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