Selected article for: "cell culture and western blot analysis"
Author: Klaile, Esther; Klassert, Tilman E; Scheffrahn, Inka; Müller, Mario M; Heinrich, Annina; Heyl, Kerstin A; Dienemann, Hendrik; Grünewald, Christiane; Bals, Robert; Singer, Bernhard B; Slevogt, Hortense
Title: Carcinoembryonic antigen (CEA)-related cell adhesion molecules are co-expressed in the human lung and their expression can be modulated in bronchial epithelial cells by non-typable Haemophilus influenzae, Moraxella catarrhalis, TLR3, and type I and II interferons
  • Document date: 2013_8_14
    • ID: 1fmsipqu_41
    Snippet: BALF samples with at an average concentration of 93 ng/ ml (± 48 ng/ml SD). In order to approach soluble CEACAM1 expression in human lung with an alternative method, normal human bronchial epithelial (NHBE) cells from healthy donors were used for transcription analysis. RT-PCR and subsequent sequencing of the obtained PCRproducts confirmed the expression of three soluble CEACAM1 isoforms, CEACAM1-4C1, CEACAM1-3 and CEACAM1-3C2 ( Figure 2B ). How.....
    Document: BALF samples with at an average concentration of 93 ng/ ml (± 48 ng/ml SD). In order to approach soluble CEACAM1 expression in human lung with an alternative method, normal human bronchial epithelial (NHBE) cells from healthy donors were used for transcription analysis. RT-PCR and subsequent sequencing of the obtained PCRproducts confirmed the expression of three soluble CEACAM1 isoforms, CEACAM1-4C1, CEACAM1-3 and CEACAM1-3C2 ( Figure 2B ). However, mRNAs of all three isoforms were not expressed at sufficient levels to allow for reliable quantification analysis by qPCR. To test for expressed and shedded soluble CEACAMs in NHBE cells, CEACAM1-, 5-, and 6-immunoprecipitations from cell culture supernatants were analyzed. Western blot analysis revealed soluble CEACAM6 and low amounts of soluble CEACAM1, but no soluble CEACAM5 ( Figure 2C ) in NHBE cell culture supernatants.

    Search related documents:
    Co phrase search for related documents
    • alternative method and cell culture: 1, 2
    • average concentration and bronchial epithelial: 1
    • average concentration and cell culture: 1
    • average concentration and human bronchial epithelial: 1
    • average concentration and human lung: 1, 2
    • BALF sample and cell culture: 1, 2
    • BALF sample and cell culture supernatant: 1
    • blot analysis and bronchial epithelial: 1, 2, 3, 4, 5, 6
    • blot analysis and cell culture: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20
    • blot analysis and cell culture supernatant: 1
    • blot analysis and human bronchial epithelial: 1, 2, 3
    • blot analysis and human lung: 1, 2, 3, 4, 5
    • blot analysis and NHBE cell: 1
    • bronchial epithelial and CEACAM1 expression: 1
    • bronchial epithelial and cell culture: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
    • bronchial epithelial and healthy donor: 1
    • bronchial epithelial and human bronchial epithelial: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • bronchial epithelial and human lung: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • bronchial epithelial and NHBE cell: 1, 2, 3, 4, 5, 6