Selected article for: "cell culture and equal volume"
Author: Hu, Tingsong; Qiu, Wei; He, Biao; Zhang, Yan; Yu, Jing; Liang, Xiu; Zhang, Wendong; Chen, Gang; Zhang, Yingguo; Wang, Yiyin; Zheng, Ying; Feng, Ziliang; Hu, Yonghe; Zhou, Weiguo; Tu, Changchun; Fan, Quanshui; Zhang, Fuqiang
Title: Characterization of a novel orthoreovirus isolated from fruit bat, China
  • Document date: 2014_11_30
    • ID: 1fw9ng2p_35
    Snippet: Serial 2-fold dilutions (1:10 to 1:640) of control and fruit bat sera were prepared in duplicate. An equal volume of Cangyuan virus working stock containing 150 TCID50 was added to the diluted sera and incubated for 1 hour. The preincubated virus/serum mix was added to 80-90% confluent Vero cell monolayers. After 1 hour of virus adsorption, the inoculum was removed, the monolayers washed three times with 2%FCS MEM, and fresh media added. All incu.....
    Document: Serial 2-fold dilutions (1:10 to 1:640) of control and fruit bat sera were prepared in duplicate. An equal volume of Cangyuan virus working stock containing 150 TCID50 was added to the diluted sera and incubated for 1 hour. The preincubated virus/serum mix was added to 80-90% confluent Vero cell monolayers. After 1 hour of virus adsorption, the inoculum was removed, the monolayers washed three times with 2%FCS MEM, and fresh media added. All incubations were performed under standard cell culture conditions. Vero cell monolayers were observed for CPE 3 days post-inoculation. The ability of sera to neutralize virus was determined by scoring the extent of CPE observed for duplicate wells.

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