Chemical Constituents and an Antineuroinflammatory Lignan, Savinin from the Roots of Acanthopanax henryi

Li, Xiao-Jun; Kim, Kwan-Woo; Oh, Hyuncheol; Liu, Xiang-Qian; Kim, Youn-Chul

. BV2 cells were cultured in 24-well culture plates at a density of 5 × 10 4 cells/well. BV2 cells were pretreated with the isolated compounds from A. henryi and then stimulated with LPS (1 g/mL) for 24 h. After incubation, 100 L of each supernatant was collected and mixed with the same volume of the Griess reagent. The absorbance at 540 nm wavelength was measured using a microplate reader. The detailed procedures are described in our previous report [14] . 2 Assay. The level of PGE 2 present in each sample was determined using a commercially available kit from R&D Systems (Minneapolis, MN, USA). Three independent assays were performed according to the manufacturer's instructions. Briefly, BV2 cells were seeded in 24-well culture plates at a density of 5 × 10 4 cells/well. Prior to the stimulation with LPS (1 g/mL) for 24 h, cells were treated with test compounds. After incubation, supernatant was collected and applied to the PGE 2 ELISA kit for measuring the concentration of PGE 2 .