Author: Zhai, Xiaofeng; Wang, Shilei; Zhu, Mengyan; He, Wei; Pan, Zhongzhou; Su, Shuo
Title: Antiviral Effect of Lithium Chloride and Diammonium Glycyrrhizinate on Porcine Deltacoronavirus In Vitro Document date: 2019_9_9
ID: 1m5yr8uc_15
Snippet: After infection, the cells were fixed with cold polysorbate for 30 min at room temperature and permeabilized with 0.2% Triton X-100 in PBS for 10 min. After washing five times with PBS, cthe ells were incubated with rabbit anti-N antibody (1:500) at 4 • C for 12 h. The cells were then incubated with FITC-conjugated goat anti-rabbit IgG (1:100, KPL, Colton, CA, USA) at 37 • C for 30 min. After washing five times with PBS, the cells were incuba.....
Document: After infection, the cells were fixed with cold polysorbate for 30 min at room temperature and permeabilized with 0.2% Triton X-100 in PBS for 10 min. After washing five times with PBS, cthe ells were incubated with rabbit anti-N antibody (1:500) at 4 • C for 12 h. The cells were then incubated with FITC-conjugated goat anti-rabbit IgG (1:100, KPL, Colton, CA, USA) at 37 • C for 30 min. After washing five times with PBS, the cells were incubated with DAPI (1:1000, diluted in PBS) at room temperature for 20 min. Fluorescence was observed under a Nikon ECLIPSE TS100 fluorescence microscope (Nikon, Tokyo, Japan).
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