Selected article for: "essential medium and MEM essential medium"
Author: Jiang, Yuting; Zhao, Guangyu; Song, Nianping; Li, Pei; Chen, Yuehong; Guo, Yan; Li, Junfeng; Du, Lanying; Jiang, Shibo; Guo, Renfeng; Sun, Shihui; Zhou, Yusen
Title: Blockade of the C5a–C5aR axis alleviates lung damage in hDPP4-transgenic mice infected with MERS-CoV
  • Document date: 2018_4_24
    • ID: 0kihygau_20
    Snippet: The lung tissues of infected mice were harvested aseptically at the indicated time points and homogenized in minimal essential medium (MEM) plus antibiotics to produce 10% (w/v) suspensions. Tissue homogenates were centrifuged and titered on monolayers of Vero cells. The cytopathic effects (CPEs) were observed daily via phase-contrast microscopy for 3 days. The viral titers were determined as TCID 50 using a CPE-based assay and calculated using t.....
    Document: The lung tissues of infected mice were harvested aseptically at the indicated time points and homogenized in minimal essential medium (MEM) plus antibiotics to produce 10% (w/v) suspensions. Tissue homogenates were centrifuged and titered on monolayers of Vero cells. The cytopathic effects (CPEs) were observed daily via phase-contrast microscopy for 3 days. The viral titers were determined as TCID 50 using a CPE-based assay and calculated using the Reed and Muench method. The viral titer was expressed as Log 10 TCID 50 /g of lung tissue.

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