Selected article for: "body weight and MERS cov"
Author: Jiang, Yuting; Zhao, Guangyu; Song, Nianping; Li, Pei; Chen, Yuehong; Guo, Yan; Li, Junfeng; Du, Lanying; Jiang, Shibo; Guo, Renfeng; Sun, Shihui; Zhou, Yusen
Title: Blockade of the C5a–C5aR axis alleviates lung damage in hDPP4-transgenic mice infected with MERS-CoV
  • Document date: 2018_4_24
    • ID: 0kihygau_8
    Snippet: Six-week-old female hDPP4-Tg mice 27 were maintained in a pathogen-free facility and housed in cages containing sterilized feed and drinking water. MERS-CoV (HCoV-EMC2012 strain) was propagated and titrated on Vero cells. Following intraperitoneal anesthetization with sodium pentobarbital (5 mg/kg of body weight), mice were treated intravenously (600 μg/kg, i.v.) with a monoclonal Ab (mAb) against mouse C5aR (Hycult Biotech, The Netherlands) or .....
    Document: Six-week-old female hDPP4-Tg mice 27 were maintained in a pathogen-free facility and housed in cages containing sterilized feed and drinking water. MERS-CoV (HCoV-EMC2012 strain) was propagated and titrated on Vero cells. Following intraperitoneal anesthetization with sodium pentobarbital (5 mg/kg of body weight), mice were treated intravenously (600 μg/kg, i.v.) with a monoclonal Ab (mAb) against mouse C5aR (Hycult Biotech, The Netherlands) or phosphate-buffered saline (PBS) as a sham treatment control. All mice were then intranasally inoculated with MERS-CoV (10 3.3 50% tissue culture infectious dose (TCID 50 )) in 20 μl Dulbecco's modified Eagle's medium. All infectious experiments related to MERS-CoV were performed in an approved biosafety level 3 facility.

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