Author: Li, Xiao-Jun; Kim, Kwan-Woo; Oh, Hyuncheol; Liu, Xiang-Qian; Kim, Youn-Chul
Title: Chemical Constituents and an Antineuroinflammatory Lignan, Savinin from the Roots of Acanthopanax henryi Document date: 2019_2_21
ID: 1vbkttzx_8
Snippet: The n-BuOH extract (100 g) was subjected to a C 18 CC (7.0 × 60 cm) elution with a gradient of MO) . Primary antibodies such as anti-inducible nitric oxide synthase (iNOS) and anti-cyclooxygenase-2 (COX-2) antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Anti-pextracellular signal-regulated kinase (ERK), anti-ERK, antip-c-Jun N-terminal kinase (JNK), anti-JNK, anti-p-p38, and anti-p38 were obtained from Cell Signaling T.....
Document: The n-BuOH extract (100 g) was subjected to a C 18 CC (7.0 × 60 cm) elution with a gradient of MO) . Primary antibodies such as anti-inducible nitric oxide synthase (iNOS) and anti-cyclooxygenase-2 (COX-2) antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Anti-pextracellular signal-regulated kinase (ERK), anti-ERK, antip-c-Jun N-terminal kinase (JNK), anti-JNK, anti-p-p38, and anti-p38 were obtained from Cell Signaling Technology (Danvers, MA, USA). Anti-mouse, anti-goat, and anti-rabbit secondary antibodies were supplied by Merck Millipore (Darmstadt, Germany). The enzyme-linked immunosorbent assay (ELISA) kit for PGE 2 was purchased from R&D Systems, Inc. (Minneapolis, MN, USA).
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