Chemical screen identifies a geroprotective role of quercetin in premature aging

Geng, Lingling; Liu, Zunpeng; Zhang, Weiqi; Li, Wei; Wu, Zeming; Wang, Wei; Ren, Ruotong; Su, Yao; Wang, Peichang; Sun, Liang; Ju, Zhenyu; Chan, Piu; Song, Moshi; Qu, Jing; Liu, Guang-Hui

Besides WS, Hutchinson-Gilford progeria syndrome (HGPS) hMSCs are another premature senescent stem cell model we recently established by introducing heterozygous Quantitative data on the right are shown as mean ± SEM (cell number ≥ 300). ***P < 0.001 vs. Ctrl. Scale bar, 10 μm. (C) Immunoblotting analysis of LAP2β and HP1γ in vehicle-and Que-treated WS hMSCs (passage 7). β-Actin was used as the loading control. Quantitative data are shown as mean ± SEM (n = 3). *P < 0.05 vs. Ctrl; ***P < 0.001 vs. Ctrl. (D) Visualization of heterochromatin architecture of vehicle-and Que-treated WS hMSCs (passage 7) by transmission electron microscope (TEM). Highmagnification views of heterochromatin around nuclear envelope are shown on the right. The percentage of cells with sparse heterochromatin at nuclear periphery is indicated at the lower left corner of each representative image. Cell number > 100. Scale bar, 2 μm.