Chemical screen identifies a geroprotective role of quercetin in premature aging

Geng, Lingling; Liu, Zunpeng; Zhang, Weiqi; Li, Wei; Wu, Zeming; Wang, Wei; Ren, Ruotong; Su, Yao; Wang, Peichang; Sun, Liang; Ju, Zhenyu; Chan, Piu; Song, Moshi; Qu, Jing; Liu, Guang-Hui

Differentiation of hESCs into mesenchymal stem cells (MSCs) was performed as described previously (Duan et al., 2015; Fu et al., 2016; Wang et al., 2018a; Wang et al., 2018b; Zhang et al., 2015) . Briefly, embryoid bodies were cultured in matrigel-coated plates with hMSC differentiation medium (α-MEM with GlutaMAX (Gibco), 10% FBS (Gemcell, Lot A77E01F), 10 ng/mL FGF2 (Joint Protein Central), 5 ng/mL TGFβ (Stemimmune LLC) and 1% penicillin/streptomycin (Gibco)). After about 10 days, the confluent MSC-like cells were passaged on gelatin-coated plate and cultured in hMSC culture medium containing 90% α-MEM with Glutamax (Gibco), 10% fetal bovine serum (FBS, Gemcell, Lot A77E01F), 1 ng/mL FGF2 (Joint Protein Central) and 1% penicillin/streptomycin (Gibco). (E) Cell apoptosis assay in vehicle-and Que-treated RS hMSCs (passage 13). Quantitative data on the right are shown as mean ± SEM (n = 3). *P < 0.05 vs. Ctrl.