Selected article for: "Bio rad and real time system"
Author: Geng, Lingling; Liu, Zunpeng; Zhang, Weiqi; Li, Wei; Wu, Zeming; Wang, Wei; Ren, Ruotong; Su, Yao; Wang, Peichang; Sun, Liang; Ju, Zhenyu; Chan, Piu; Song, Moshi; Qu, Jing; Liu, Guang-Hui
Title: Chemical screen identifies a geroprotective role of quercetin in premature aging
  • Document date: 2018_8_1
    • ID: 1mrj6nb3_63
    Snippet: Total RNAs were extracted using TRIzol Reagent (Invitrogen). 1-2 μg of total RNAs were converted to cDNA by using GoScript Reverse Transcription System (Promega). Quantitative real-time PCR was performed with iTaq Universal SYBR Green Super mix (Bio-Rad) on a CFX384 Real-Time PCR system (Bio-Rad). Data were normalized to 18S rRNA transcript and calculated using the ΔΔCq method. Quantitative RT-PCR primers used were listed in Table S4 ......
    Document: Total RNAs were extracted using TRIzol Reagent (Invitrogen). 1-2 μg of total RNAs were converted to cDNA by using GoScript Reverse Transcription System (Promega). Quantitative real-time PCR was performed with iTaq Universal SYBR Green Super mix (Bio-Rad) on a CFX384 Real-Time PCR system (Bio-Rad). Data were normalized to 18S rRNA transcript and calculated using the ΔΔCq method. Quantitative RT-PCR primers used were listed in Table S4 .

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