Application and optimization of RT-PCR in diagnosis of SARS-CoV-2 infection

Guanmin Jiang; Xiaoshuai Ren; Yan Liu; Hongtao Chen; Wei Liu; Zhaowang Guo; Yaqin Zhang; Chaoqun Chen; Jianhui Zhou; Qiang Xiao; Hong Shan

is the (which was not peer-reviewed) The copyright holder for this preprint . https://doi.org/10.1101/2020.02.25.20027755 doi: medRxiv preprint Given that the time on receiving detection can cause influences on sensitivity, the time differences between RT-PCR for pharyngeal and CT scan were compared. The average time of nucleic acid screening was earlier than that of CT scan statistically (-0.7390days, -3625~1.674) ( Table 4) It is notable that both of the methods acquired the sensitivity less than 80% in screening SARS-CoV-22 infections, which is not ideal enough for the diagnosis of infectious diseases with severe consequences. To develop more appropriate detection scheme, the performances of combination . CC-BY-ND 4.0 International license It is made available under a author/funder, who has granted medRxiv a license to display the preprint in perpetuity. . CC-BY-ND 4.0 International license It is made available under a author/funder, who has granted medRxiv a license to display the preprint in perpetuity.