Selected article for: "fluorescence intensity and MFI mean fluorescence intensity"
Author: Delvecchio, Rodrigo; Higa, Luiza M.; Pezzuto, Paula; Valadão, Ana Luiza; Garcez, Patrícia P.; Monteiro, Fábio L.; Loiola, Erick C.; Dias, André A.; Silva, Fábio J. M.; Aliota, Matthew T.; Caine, Elizabeth A.; Osorio, Jorge E.; Bellio, Maria; O’Connor, David H.; Rehen, Stevens; de Aguiar, Renato Santana; Savarino, Andrea; Campanati, Loraine; Tanuri, Amilcar
Title: Chloroquine, an Endocytosis Blocking Agent, Inhibits Zika Virus Infection in Different Cell Models
  • Document date: 2016_11_29
    • ID: 01s21vh0_15
    Snippet: Vero, hBMEC, and NSC were exposed to ZIKV MR 766 at an MOI of 2. After 1 h, inoculum was removed and chloroquine-containing medium (6.25 to 50 µM) was added to the cells. Five days post-infection, 20 µL of CellTiter Blue reagent (Promega, Madison, WI, USA) was added in each well, incubated for 12-16 h, and fluorescence was measured (560/590 nm), except for NSCp14 cells when CellTiter Blue was added at three days post-infection. Mean fluorescenc.....
    Document: Vero, hBMEC, and NSC were exposed to ZIKV MR 766 at an MOI of 2. After 1 h, inoculum was removed and chloroquine-containing medium (6.25 to 50 µM) was added to the cells. Five days post-infection, 20 µL of CellTiter Blue reagent (Promega, Madison, WI, USA) was added in each well, incubated for 12-16 h, and fluorescence was measured (560/590 nm), except for NSCp14 cells when CellTiter Blue was added at three days post-infection. Mean fluorescence intensity (MFI) and standard deviation were displayed. In order to calculate the half maximum effective concentration (EC50) that protects cells from death caused by ZIKV infection, MFI values from ZIKV MR766 control were subtracted from every condition and then these values were normalized over the mock control. These data were plotted and Hill 4 parameter sigmoidal regression was performed on Sigma Plot v.12.0 (Systat Software Inc., San Jose, CA, USA).

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