Author: Perdomo, German; Dong, H. Henry
Title: Apolipoprotein D in Lipid Metabolism and Its Functional Implication in Atherosclerosis and Aging Document date: 2008_12_12
ID: 167z915s_40
Snippet: Immunohistochemistry. Mice were sacrificed and the proximal aorta of individual mice was dissected free of adipose and connective tissue, and immediately fixed in 4% paraformaldehyde. The aorta was mounted in Cryomatrix (Shandon, Pittsburgh, PA) and frozen in isopentane that has been pre-cooled in liquid nitrogen. Transverse cryo-sections (10 µm) were cut and stained by oil red O to visualize the atherosclerotic lesions. Consecutive sections wer.....
Document: Immunohistochemistry. Mice were sacrificed and the proximal aorta of individual mice was dissected free of adipose and connective tissue, and immediately fixed in 4% paraformaldehyde. The aorta was mounted in Cryomatrix (Shandon, Pittsburgh, PA) and frozen in isopentane that has been pre-cooled in liquid nitrogen. Transverse cryo-sections (10 µm) were cut and stained by oil red O to visualize the atherosclerotic lesions. Consecutive sections were immunostained using either rabbit control IgG derived against bacterial βgalactosidase or polyclonal rabbit anti-apoD, followed by incubation with the donkey anti-rabbit IgG conjugated with Cy3. All animal studies were approved by the IACUC of Children's Hospital of Pittsburgh (protocol #30-07).
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