Author: Labrie, Marilyne; Lalonde, Simon; Najyb, Ouafa; Thiery, Maxime; Daneault, Caroline; Des Rosiers, Chrisitne; Rassart, Eric; Mounier, Catherine
Title: Apolipoprotein D Transgenic Mice Develop Hepatic Steatosis through Activation of PPAR? and Fatty Acid Uptake Document date: 2015_6_17
ID: 0wtq1c15_18
Snippet: Liver samples were incubated overnight at 4°C in 4% paraformaldehyde, frozen in dry ice and kept at −80°C until further use. 4 microns thick longitudinal sections were sliced with a cryostat and incubated 5 minutes in a solution of PBS containing 0.04 mg/ml propidium iodide and 0.1 μg/ml Bodipy 493/503. After 3 washes of 5 min in PBS, coverslips were mounted onto slides using Prolong Gold antifade reagent and observed within 24h with a laser.....
Document: Liver samples were incubated overnight at 4°C in 4% paraformaldehyde, frozen in dry ice and kept at −80°C until further use. 4 microns thick longitudinal sections were sliced with a cryostat and incubated 5 minutes in a solution of PBS containing 0.04 mg/ml propidium iodide and 0.1 μg/ml Bodipy 493/503. After 3 washes of 5 min in PBS, coverslips were mounted onto slides using Prolong Gold antifade reagent and observed within 24h with a laser scanning confocal microscope (Nikon TE300) (original magnification X60). Lipid droplets were visualized and quantified using image J software.
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