Chemical Constituents and an Antineuroinflammatory Lignan, Savinin from the Roots of Acanthopanax henryi

Li, Xiao-Jun; Kim, Kwan-Woo; Oh, Hyuncheol; Liu, Xiang-Qian; Kim, Youn-Chul

in LPS-Induced BV2 Microglial Cells. Among the isolated compounds from A. henryi, we selected 13 compounds (compounds -, , , , , , , , and -) based on their number of publications, and these compounds were screened for anti-inflammatory effects, including the inhibition of NO and PGE 2 production, in LPS-stimulated BV2 microglial cells. BV2 cells were pretreated with the compounds for 3 h and stimulated with LPS (1 g/mL) for 24 h. The concentration required to inhibit the production of NO by 50% (IC 50 value) was calculated based on the concentrations of NO and PGE 2 released into the culture Figure 2 : The effect of compound on LPS-induced iNOS and COX-2 protein expression in BV2 microglial cells. Cells were pretreated with/without the indicated concentrations of compound for 3 h and then stimulated with LPS (1 g/mL) for 24 h. The levels of iNOS and COX-2 were determined by Western blot analysis. The experiment was repeated three times, and similar results were obtained. media as measured by the Griess method and PGE 2 ELISA kit, respectively. Among the tested compounds, only compound showed inhibitory effects against LPS-induced NO production with an IC 50 value of 2.22 ± 0.11 M and LPSinduced PGE 2 production with an IC 50 value of 2.28 ± 0.23 M. The IC 50 values of butein, a positive control, were 4.41 ± 0.45 M in NO production and 3.26 ± 0.53 M in PGE 2 production, respectively. At tested concentrations, all test compounds did not show cytotoxic effects to BV2 cells (data not shown). Therefore, compound was further investigated to elucidate the up-stream signaling pathways of its antineuroinflammatory effect.