Author: Delvecchio, Rodrigo; Higa, Luiza M.; Pezzuto, Paula; Valadão, Ana Luiza; Garcez, Patrícia P.; Monteiro, Fábio L.; Loiola, Erick C.; Dias, André A.; Silva, Fábio J. M.; Aliota, Matthew T.; Caine, Elizabeth A.; Osorio, Jorge E.; Bellio, Maria; O’Connor, David H.; Rehen, Stevens; de Aguiar, Renato Santana; Savarino, Andrea; Campanati, Loraine; Tanuri, Amilcar
Title: Chloroquine, an Endocytosis Blocking Agent, Inhibits Zika Virus Infection in Different Cell Models Document date: 2016_11_29
ID: 01s21vh0_21
Snippet: Vero cells were infected with ZIKV MR766 or ZIKV BR strain at an MOI of 2 for 1 h at 4 • C. Virus input was washed three times with cold PBS and cells were treated with chloroquine (6.25 to 50 µM) for 48 h, and then the supernatant was collected and the RNA was extracted and analyzed by relative quantification by RT-qPCR. The supernatant was also evaluated by plaque assay to quantitate the infectious virus particles......
Document: Vero cells were infected with ZIKV MR766 or ZIKV BR strain at an MOI of 2 for 1 h at 4 • C. Virus input was washed three times with cold PBS and cells were treated with chloroquine (6.25 to 50 µM) for 48 h, and then the supernatant was collected and the RNA was extracted and analyzed by relative quantification by RT-qPCR. The supernatant was also evaluated by plaque assay to quantitate the infectious virus particles.
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