Carcinoembryonic antigen (CEA)-related cell adhesion molecules are co-expressed in the human lung and their expression can be modulated in bronchial epithelial cells by non-typable Haemophilus influenzae, Moraxella catarrhalis, TLR3, and type I and II interferons

Klaile, Esther; Klassert, Tilman E; Scheffrahn, Inka; Müller, Mario M; Heinrich, Annina; Heyl, Kerstin A; Dienemann, Hendrik; Grünewald, Christiane; Bals, Robert; Singer, Bernhard B; Slevogt, Hortense

Preparation of aqueous phase cigarette smoke extract (CSE) CSE was prepared as described [37] . Briefly, smoke from one cigarette (10 mg Tar, 0.8 mg nicotine; Camel Filters, Japan Tobaco International) with the mouthpiece filter removed was bubbled through 10 ml complete medium using a peristaltic pump (P-1, GE Healthcare) and filtered through a 0.22 μm filter. Suction was regulated, so that sidestream smoke developed during the entire combustion, lasting 5 min for each cigarette. The filtered CSE was regarded as 100%. CSE was used within 30 min of preparation at a final concentration of 4%. Lau et al. showed that NHBE cells were induced to secrete IL-8 and remained viable after tratment with 4% CSE for 24 h [37] .