Author: Hu, Tingsong; Qiu, Wei; He, Biao; Zhang, Yan; Yu, Jing; Liang, Xiu; Zhang, Wendong; Chen, Gang; Zhang, Yingguo; Wang, Yiyin; Zheng, Ying; Feng, Ziliang; Hu, Yonghe; Zhou, Weiguo; Tu, Changchun; Fan, Quanshui; Zhang, Fuqiang
Title: Characterization of a novel orthoreovirus isolated from fruit bat, China Document date: 2014_11_30
ID: 1fw9ng2p_22
Snippet: Fifty fruit bats (Rousettus leschenaultia) were collected from across eight different locations in Cangyuan city of China's Yunnan province. All of the locations including three lychee, two mango orchards and three caverns were in close proximity to human residences. All bats were euthanized by intracardiac injection of sodium pentobarbital. Following euthanasia, serum samples were collected by cardiac puncture. The serum samples were used for ne.....
Document: Fifty fruit bats (Rousettus leschenaultia) were collected from across eight different locations in Cangyuan city of China's Yunnan province. All of the locations including three lychee, two mango orchards and three caverns were in close proximity to human residences. All bats were euthanized by intracardiac injection of sodium pentobarbital. Following euthanasia, serum samples were collected by cardiac puncture. The serum samples were used for neutralizing-antibody tests. Necropsies were performed directly following euthanasia. The necropsy findings demonstrated that these bats were apparently healthy and no special pathological lesions was observed (data not show). The intestine samples were used for virus isolation and the kidney, heart, lung, liver, spleen, rectal swab and brain samples were processed for PCRs testing. The tissue samples stored at -80°C.
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