Author: Geng, Lingling; Liu, Zunpeng; Zhang, Weiqi; Li, Wei; Wu, Zeming; Wang, Wei; Ren, Ruotong; Su, Yao; Wang, Peichang; Sun, Liang; Ju, Zhenyu; Chan, Piu; Song, Moshi; Qu, Jing; Liu, Guang-Hui
Title: Chemical screen identifies a geroprotective role of quercetin in premature aging Document date: 2018_8_1
ID: 1mrj6nb3_51
Snippet: The human MSCs were purified by sorting against CD73, CD90 and CD105 triple positive cells using FACS. In details, hMSCs were digested using TrypLE Express (Gibco) and washed twice by PBS. Cells were incubated with primary antibody diluted with 10% FBS in PBS for 30 min at room temperature and sorted by using flow cytometer (BD FACSAria IIIu)......
Document: The human MSCs were purified by sorting against CD73, CD90 and CD105 triple positive cells using FACS. In details, hMSCs were digested using TrypLE Express (Gibco) and washed twice by PBS. Cells were incubated with primary antibody diluted with 10% FBS in PBS for 30 min at room temperature and sorted by using flow cytometer (BD FACSAria IIIu).
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