Selected article for: "cell surface expression and FACS analysis"
Author: Klaile, Esther; Klassert, Tilman E; Scheffrahn, Inka; Müller, Mario M; Heinrich, Annina; Heyl, Kerstin A; Dienemann, Hendrik; Grünewald, Christiane; Bals, Robert; Singer, Bernhard B; Slevogt, Hortense
Title: Carcinoembryonic antigen (CEA)-related cell adhesion molecules are co-expressed in the human lung and their expression can be modulated in bronchial epithelial cells by non-typable Haemophilus influenzae, Moraxella catarrhalis, TLR3, and type I and II interferons
  • Document date: 2013_8_14
    • ID: 1fmsipqu_47
    Snippet: It was then tested whether the up-regulation of CEACAM1 transcripts upon treatment with IFNα, IFNβ, IFNγ, and poly I:C were also reflected by higher cell surface protein levels. FACS analysis confirmed an increased expression of CEACAM1 on the cell surface of NHBE cells under all four conditions ( Figure 4B ). The CEACAM1-specific antibody used for flow cytometry did not allow discrimination between the different isoforms......
    Document: It was then tested whether the up-regulation of CEACAM1 transcripts upon treatment with IFNα, IFNβ, IFNγ, and poly I:C were also reflected by higher cell surface protein levels. FACS analysis confirmed an increased expression of CEACAM1 on the cell surface of NHBE cells under all four conditions ( Figure 4B ). The CEACAM1-specific antibody used for flow cytometry did not allow discrimination between the different isoforms.

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