Author: Perra, Léa; Balloy, Viviane; Foussignière, Tobias; Moissenet, Didier; Petat, Hortense; Mungrue, Imran N.; Touqui, Lhousseine; Corvol, Harriet; Chignard, Michel; Guillot, Loic
Title: CHAC1 Is Differentially Expressed in Normal and Cystic Fibrosis Bronchial Epithelial Cells and Regulates the Inflammatory Response Induced by Pseudomonas aeruginosa Document date: 2018_11_29
ID: 10fe70kl_13
Snippet: RNA was isolated using a NucleoSpin RNA/Protein kit (Macherey Nagel, Duren, Germany). RT was performed using a high-capacity cDNA kit (Applied Biosystems, Foster City, CA, USA). Real-time qPCR was performed with an ABI StepOnePlus, using TaqMan Fast Universal PCR Master Mix (Applied Biosystems), TaqMan probes for CHAC1 (Hs_00225520), IL-8 (Hs_174103), IL-6 (Hs_985639), CCL2 (Hs_00234140), GAPDH (Hs_2786624) and cDNA as a template. For relative qu.....
Document: RNA was isolated using a NucleoSpin RNA/Protein kit (Macherey Nagel, Duren, Germany). RT was performed using a high-capacity cDNA kit (Applied Biosystems, Foster City, CA, USA). Real-time qPCR was performed with an ABI StepOnePlus, using TaqMan Fast Universal PCR Master Mix (Applied Biosystems), TaqMan probes for CHAC1 (Hs_00225520), IL-8 (Hs_174103), IL-6 (Hs_985639), CCL2 (Hs_00234140), GAPDH (Hs_2786624) and cDNA as a template. For relative quantification, the amount of target genes was normalized to the expression of GAPDH relative to control cells used as a calibrator and was calculated using the 2 − Ct method.
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