Author: Lau, Yee-Ling; Lai, Meng-Yee; Teoh, Boon-Teong; Abd-Jamil, Juraina; Johari, Jefree; Sam, Sing-Sin; Tan, Kim-Kee; AbuBakar, Sazaly
Title: Colorimetric Detection of Dengue by Single Tube Reverse-Transcription-Loop-Mediated Isothermal Amplification Document date: 2015_9_18
ID: 1946zslt_18
Snippet: For sensitivity assessment, plasmids containing the target region of the 3 0 -NCR gene were constructed for each serotype for use in the LAMP reaction. The target DNA sequence was amplified with 2 LAMP primers (F3 and B3 primers) by PCR and was then cloned into the pGEM-T cloning vector (Promega, USA). Plasmid DNA purification was performed with a QIAprep Miniprep kit (QIAGEN, Hilden, Germany). The resulting sequences were aligned using the 3 0 -.....
Document: For sensitivity assessment, plasmids containing the target region of the 3 0 -NCR gene were constructed for each serotype for use in the LAMP reaction. The target DNA sequence was amplified with 2 LAMP primers (F3 and B3 primers) by PCR and was then cloned into the pGEM-T cloning vector (Promega, USA). Plasmid DNA purification was performed with a QIAprep Miniprep kit (QIAGEN, Hilden, Germany). The resulting sequences were aligned using the 3 0 -NCR gene sequences for DENV1-4 in GenBank to confirm that the target sequences were correct.
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