Selected article for: "copy number and target copy number"
Author: Lau, Yee-Ling; Lai, Meng-Yee; Teoh, Boon-Teong; Abd-Jamil, Juraina; Johari, Jefree; Sam, Sing-Sin; Tan, Kim-Kee; AbuBakar, Sazaly
Title: Colorimetric Detection of Dengue by Single Tube Reverse-Transcription-Loop-Mediated Isothermal Amplification
  • Document date: 2015_9_18
    • ID: 1946zslt_20
    Snippet: Positive control plasmid DNAs were used to determine the minimum copy number (lower detection limit) of the target gene sequence detectable by LAMP. The standard curve for LAMP was constructed using 10-fold serial dilutions of plasmid DNA (106 copies to 1 copy) to sterile water. DENV RNA was extracted from the culture supernatant DENV-1 genotype I, II, III; DENV-2, Asian I and cosmopolitan; DENV-3, genotype I, II and III and DENV-4, sub genotype .....
    Document: Positive control plasmid DNAs were used to determine the minimum copy number (lower detection limit) of the target gene sequence detectable by LAMP. The standard curve for LAMP was constructed using 10-fold serial dilutions of plasmid DNA (106 copies to 1 copy) to sterile water. DENV RNA was extracted from the culture supernatant DENV-1 genotype I, II, III; DENV-2, Asian I and cosmopolitan; DENV-3, genotype I, II and III and DENV-4, sub genotype IIa and IIb) [8, 9, 10, 11] . The sensitivity of RT-LAMP assay was assessed using a panel of serially diluted viral RNA (1000, 100, 10, and 1 copy numbers) extracted from culture supernatant. The viral RNA used was quantitated using the genesig Real-Time qRT-PCR DENV

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