Characterization of murine CEACAM1 in vivo reveals low expression on CD8(+) T cells and no tumor growth modulating activity by anti-CEACAM1 mAb CC1

McLeod, Robbie L.; Angagaw, Minilik H.; Baral, Toya Nath; Liu, Liming; Moniz, Raymond Joseph; Laskey, Jason; Hsieh, SuChun; Lee, Mike; Han, Jin-Hwan; Issafras, Hassan; Javaid, Sarah; Loboda, Andrey; Sadekova, Svetlana; O'Connor, Joann A.; Tse, Archie; Punnonen, Juha

CT26 tumor cells were cloned from N-nitroso-Nmethylurethane (NNMU)-induced, undifferentiated colon carcinoma cell line. MBT-2 was initiated from primary N-[4-(5-nitro-2-furyl)-2-thiazolyl] formamide (FANFT) induced murine bladder tumors arising in C3H/He mice. CT26 and MBT-2 cells were cultured in RPMI-1640 and DMEM medium respectively containing 10% fetal bovine serum. Both mouse colon adenocarcinoma cell-line, MC38, and a mouse bladder carcinoma cell-line, MB49 were cultured in DMEM medium with 10% fetal bovine serum. The tumor cells were then grown in tissue culture flasks in a humidified incubator at 37° C at atmosphere conditions of 5% CO2 and 95% air. A20 cell line is a BALB/c B cell lymphoma line derived from a spontaneous reticulum cell neoplasm. The cells were cultured in RPMI-1640 medium containing 10% fetal bovine serum and 0.05 mM 2-mercaptoethanol in tissue culture flasks as described above.