Artesunate interacts with Vitamin D receptor to reverse mouse model of sepsis-induced immunosuppression via enhancing autophagy

Shenglan Shang; Jiaqi Wu; Xiaoli Li; Xin Liu; Pan Li; Chunli Zheng; Yonghua Wang; Songqing Liu; Jiang Zheng; Hong Zhou

Lastly, based on V D3 is a natural agonist of VDR and there are a very high affinity between them (Haussler, Jurutka, Mizwicki & Norman, 2011) , the effect of AS on TNF-α level was investigated in presence and in absence of V D3 to validate the above findings. The results showed, in the absence of V D3 , TNF-α level decreased and AS-promoting TNF-α release in LPS tolerance cells. However, when pro-incubated with V D3 , the effect that . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.02.26.966143 doi: bioRxiv preprint AS-promoting TNF-α release was eliminated (Figure 3g ), indicating that there is an antagonism between V D3 and AS. These results strongly indicate that AS may bind to the same receptor or even similar sites as V D3 .