Author: Lau, Yee-Ling; Lai, Meng-Yee; Teoh, Boon-Teong; Abd-Jamil, Juraina; Johari, Jefree; Sam, Sing-Sin; Tan, Kim-Kee; AbuBakar, Sazaly
Title: Colorimetric Detection of Dengue by Single Tube Reverse-Transcription-Loop-Mediated Isothermal Amplification Document date: 2015_9_18
ID: 1946zslt_4
Snippet: Due to the ability of molecular techniques to provide diagnostic information rapidly, reverse transcription (RT)-PCR, nested PCR, nucleic acid sequence-based amplification (NASBA), and real-time PCR are used to diagnose dengue virus serotypes. The requirement to maintain reagents in cold and the need of expensive thermal cyclers (followed by gel electrophoresis) limits the utilization of these approaches [2] . However, the simplicity and high eff.....
Document: Due to the ability of molecular techniques to provide diagnostic information rapidly, reverse transcription (RT)-PCR, nested PCR, nucleic acid sequence-based amplification (NASBA), and real-time PCR are used to diagnose dengue virus serotypes. The requirement to maintain reagents in cold and the need of expensive thermal cyclers (followed by gel electrophoresis) limits the utilization of these approaches [2] . However, the simplicity and high efficiency of LAMP to rapidly amplify DNA under isothermal conditions suggested that LAMP could be a potential alternative for detecting dengue virus especially in field settings as compared to qRT-PCR which is time-consuming and require RT-PCR machine.
Search related documents:
Co phrase search for related documents- dengue virus and dna amplify: 1
- dengue virus and expensive thermal cycler: 1
- dengue virus and gel electrophoresis: 1
- dengue virus and high efficiency: 1
- dna amplify and gel electrophoresis: 1, 2
- dna amplify and high efficiency: 1, 2
- expensive thermal cycler and gel electrophoresis: 1
- gel electrophoresis and high efficiency: 1, 2, 3, 4
- high efficiency and isothermal condition: 1
Co phrase search for related documents, hyperlinks ordered by date