Author: Lau, Yee-Ling; Lai, Meng-Yee; Teoh, Boon-Teong; Abd-Jamil, Juraina; Johari, Jefree; Sam, Sing-Sin; Tan, Kim-Kee; AbuBakar, Sazaly
Title: Colorimetric Detection of Dengue by Single Tube Reverse-Transcription-Loop-Mediated Isothermal Amplification Document date: 2015_9_18
ID: 1946zslt_4
Snippet: Due to the ability of molecular techniques to provide diagnostic information rapidly, reverse transcription (RT)-PCR, nested PCR, nucleic acid sequence-based amplification (NASBA), and real-time PCR are used to diagnose dengue virus serotypes. The requirement to maintain reagents in cold and the need of expensive thermal cyclers (followed by gel electrophoresis) limits the utilization of these approaches [2] . However, the simplicity and high eff.....
Document: Due to the ability of molecular techniques to provide diagnostic information rapidly, reverse transcription (RT)-PCR, nested PCR, nucleic acid sequence-based amplification (NASBA), and real-time PCR are used to diagnose dengue virus serotypes. The requirement to maintain reagents in cold and the need of expensive thermal cyclers (followed by gel electrophoresis) limits the utilization of these approaches [2] . However, the simplicity and high efficiency of LAMP to rapidly amplify DNA under isothermal conditions suggested that LAMP could be a potential alternative for detecting dengue virus especially in field settings as compared to qRT-PCR which is time-consuming and require RT-PCR machine.
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