Author: Lau, Yee-Ling; Lai, Meng-Yee; Teoh, Boon-Teong; Abd-Jamil, Juraina; Johari, Jefree; Sam, Sing-Sin; Tan, Kim-Kee; AbuBakar, Sazaly
Title: Colorimetric Detection of Dengue by Single Tube Reverse-Transcription-Loop-Mediated Isothermal Amplification Document date: 2015_9_18
ID: 1946zslt_5
Snippet: Loop-mediated isothermal amplification (LAMP) originally developed by Notomi et al. (2000) represents a very sensitive, easy to use, and less time consuming diagnostic method [3] . LAMP can amplify up to 10 9 copies in less than1 h under isothermal conditions (65°C) using simple incubators such as water baths or heating blocks making this approach suitable for field work. The method uses 4-6 primers that recognise 6-8 distinct regions of the tar.....
Document: Loop-mediated isothermal amplification (LAMP) originally developed by Notomi et al. (2000) represents a very sensitive, easy to use, and less time consuming diagnostic method [3] . LAMP can amplify up to 10 9 copies in less than1 h under isothermal conditions (65°C) using simple incubators such as water baths or heating blocks making this approach suitable for field work. The method uses 4-6 primers that recognise 6-8 distinct regions of the target DNA which helps eliminate nonspecific binding and increases the specificity of this assay [3] .
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