Colorimetric Detection of Dengue by Single Tube Reverse-Transcription-Loop-Mediated Isothermal Amplification

Lau, Yee-Ling; Lai, Meng-Yee; Teoh, Boon-Teong; Abd-Jamil, Juraina; Johari, Jefree; Sam, Sing-Sin; Tan, Kim-Kee; AbuBakar, Sazaly

The study obtained ethics approval from the UMMC Medical Ethics Committee (Ethics Committee/IRB Reference Number: 908.11). A total of 189 serum samples from patients cl inically suspected with DENV infection were obtained from University of Malaya Medical Centre (UMMC), Kuala Lumpur. All clinical samples were taken as part of standard patient care. The samples were divided in the laboratory to be used for routine diagnosis by serological assays (SD Dengue IgM Capture and SD Dengue IgG Capture ELISA kits, Standard Diagnosis Inc., Korea) and evaluation of the RT-LAMP assay developed in this study. The viral RNA was confirmed by virus culture and genesig Real-Time qRT-PCR DENV Detection Kit (PrimerDesign Ltd., UK). As this is a retrospective study, patient samples were included in the study without any specific reference to the patient's identity. Samples collected from healthy donors (n = 24) were from Department of Parasitology, Faculty of Medicine, University of Malaya. All volunteer healthy donors were duly informed before blood was taken and written consent was obtained before the healthy donor samples were used in this study.